F(ab')2 Goat anti-rat IgG: RPE (mouse adsorbed)
Fusion Partners: Spleen cells from an immunized AUG rat were fused with cells of the mouse P3/NS1/1-Ag4.1 myeloma cell line.
Flow Cytometry: Neat-1:10
Optimal working dilutions to be determined by researcher.
Flow Cytometry: Use 10ul of the suggested working dilution to label 10e6 cells in 100ul.
| As reported|
|PVG lymphoid cells.|
|Tissue Culture Supernatant.|
|Supplied as a liquid in 0.2M Tris HCl pH 7.4, 5-10% fetal calf serum, 0.09% sodium azide.|
|Reacts with the Pta. A2 antigen which is present on the majority of peripheral T cells but only expressed at low levels in the thymus (probably by medullary thymocytes). The alloantigen is also known as Ag-F1, ART-2, RT-ly-2(5) and RT6 agreed at the 4th International Workshop on Alloantigenic Systems in the Rat, 1982. Positive on PVG, PVG-RT1a (DA), PVG-RT11 (AGUS), PVG-RT1u (AO) and PVG-R1-Negative on PVG-PtaDA rats.|
|Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.|
1. Howard, J.C., Scott, D.W. (1974) Immunology 27: 403.2. Butcher, G.W., Howard, J.C. (1977) Rat News Letter 1: 12.3. Kohler, G. and Milstein, C. Nature (1975) 256: 495.4. Kohler, G., Howe, S.C. and Milstein, C. Eu|