Description:
Fusion Partners: Spleen cell from immunized PVG-RT11 (Agus) rats were fused with cells of the Y3.Ag1.2.3 rat myeloma cell line.
Applications:
Flow Cytometry: Neat-1:4
ELISA
Radioimmunoassay
Optimal working dilutions to be determined by researcher.
Flow Cytometry: Use 50ul of the suggested working dilution to label 5x10e5 cells in 100ul.
|
| Type | Isotype | Clone | Grade |
|---|
| Mab | IgG2c | 3H450 | Supernatant |
|
| Size | | Storage | Shipping | Source | Host |
|---|
| 2ml | | -20°C | Blue Ice | Rat | Rat |
|
| Concentration: |
| As reported |
| Immunogen: |
| Spleen and lymph node cells from F344 rats. |
| Purity: |
| Tissue Culture Supernatant. |
| Form |
| Supplied as a liquid in 0.2M Tris HCl pH 7.4, 5-10% fetal calf serum, 0.09% sodium azide. |
| Specificity: |
| Detects the RT6.2 allele of the RT6 T lymphocyte marker. RT6.2 has recently been shown to possess nicotinamide adenine dinucleotide (NAD) glycohydrolase activity. |
|
| Intended for research use only. Not for use in human, therapeutic, or diagnostic applications. |
1. Butcher, G.W. (1987). A list of monoclonal antibodies specific for alloantigens of the rat. J. Immunogenetics 14: 163-176.2. Thiele, H.G. Koch, F., Hamann, A. and Arndt, R., (1986). Biochemical characterisation of the rat T cell antige
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