Fusion Partners: Spleen cell from immunized PVG-RT11 (Agus) rats were fused with cells of the Y3.Ag1.2.3 rat myeloma cell line.
Flow Cytometry: Neat-1:4
Optimal working dilutions to be determined by researcher.
Flow Cytometry: Use 50ul of the suggested working dilution to label 5x10e5 cells in 100ul.
| As reported|
|Spleen and lymph node cells from F344 rats.|
|Tissue Culture Supernatant.|
|Supplied as a liquid in 0.2M Tris HCl pH 7.4, 5-10% fetal calf serum, 0.09% sodium azide.|
|Detects the RT6.2 allele of the RT6 T lymphocyte marker. RT6.2 has recently been shown to possess nicotinamide adenine dinucleotide (NAD) glycohydrolase activity.|
|Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.|
1. Butcher, G.W. (1987). A list of monoclonal antibodies specific for alloantigens of the rat. J. Immunogenetics 14: 163-176.2. Thiele, H.G. Koch, F., Hamann, A. and Arndt, R., (1986). Biochemical characterisation of the rat T cell antige|