Technical Data
R9700-03A
Rubella, Capsid Protein (German Measles)
Description:
Rubella virus is the only member of the Rubrivirus genus of the Togavirus family. Unlike most Togaviruses it is NOT arthropod borne, but is acquired via the respiratory route. It causes German measles ( a mild contagious eruptive disease, capable of producing congenital defects in infants born to mothers infected during the first three months of pregnancy). Rubella virus is an enveloped positive-strand RNA virus. The genome encodes two open reading frames (ORFs): the 5'-proximal ORF encodes viral nonstructural proteins (NSP) that are responsible for viral genome replication, while the 3'-proximal ORF encodes three virion structural proteins (SP), the capsid protein (CP), and the two envelope glycoproteins, E2 and E1. During virus assembly, the capsid interacts with genomic RNA to form nucleocapsids. The rubella virus (RV) structural proteins: capsid, E2, and E1 are synthesized as a polyprotein precursor. The signal peptide that initiates translocation of E2 into the lumen of the endoplasmic reticulum remains attached to the carboxy terminus of the capsid protein after cleavage by signal peptidase.

Applications:
Suitable for use in Western Blot. Other applications not tested.

Recommended Dilution:
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
MabIgG1,k9L631Ascites
SizeStorageShippingSourceHost
1ml-20CBlue IceMouse
Concentration:
Not determined
Immunogen:
Rubella (C).
Purity:
Ascites
Form
Supplied as a liquid in 0.02M Boric Acid, 5mM Sodium Borate, 0.2M Sodium Chloride, pH 8.0+0.2, 0.1% Sodium azide.
Specificity:
Recognizes C protein and reacts with reduced or non-reduced antigens on Western blots. Does not have HI activity. No neutralization of infectivity.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Zhu, Z., et al., (2007), "Comparison of Four Methods Using Throat Swabs to Confirm Rubella Virus Infection", Journal of Clinical Microbiology, 45(9): 2847-2852.