Technical Data
R9700-05A
Rubella, E1 (German Measles)
Description:
Rubella virus is the only member of the Rubrivirus genus of the Togavirus family. Unlike most Togaviruses it is NOT arthropod borne, but is acquired via the respiratory route. It is an enveloped (toga=cloak), non-segmented, positive sense, RNA virus and replicates in the cytoplasm. It consists of 3 structural proteins; E1,E2 membrane bound glycoproteins and C capsid protein. E1 envelope glycoprotein is a class II viral fusion protein. Fusion activity is inactive as long as E1 is bound to E2 in mature virion. After virus attachment to target cell and endocytosis, acidification of the endosome induces dissociation of E1/E2 heterodimer and trimerization of the E1 subunits. This E1 homotrimer is fusion active, and promotes release of viral nucleocapsid in the cytoplasm after cell and viral membrane fusion. The E1 cytoplasmic tail modulates virus release, and the tyrosines residues are critical for this function.

Applications:
Suitable for use in Immunofluorescence, Neutralization and Western Blot. Other applications have not been tested.

Recommended Dilutions:
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgG2a2Q2070Affinity Purified
SizeStorageShippingSourceHost
100ug-20CBlue IceMouse
Concentration:
~1mg/ml
Immunogen:
Purity:
Purified
Form
Supplied as a liquid in PBS, pH 7.4, 0.1% sodium azide
Specificity:
Recognizes E1. Recognizes an epitope located between aa202-283 of the E1 glycoprotein.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.