Technical Data
S0050-01
S1 Nuclease, A. orzyae, ss-DNA degradation, Solution
10KU
50KU
Molecular Biology Storage: -20CShipping: Blue Ice
S1 Nuclease degrades single-stranded DNA and RNA yielding 5-phosphoryl-terminated products.

Applications:
Removal of protruding single-stranded termini in double-stranded DNA.
Selective cleavage of single-stranded DNA
Mapping RNA transcripts

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing.. Store at -20C. Aliquots are stable for at least 6 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Molecular Weight:
~34kD
Source: Aspergillus orzyae

Specificity: ss-DNA degradation. Activity on native dsDNA undetectable under the assay conditions.

Concentration: 100,000 units/ml

Form: Supplied as a liquid in 30mM sodium acetate, pH 4.6, 50mM sodium chloride, 1mM zinc chloride, 50% glycerol.

Quality Control: No double stranded DNA digestion was detected after incubation of 10units of S0050-01 with 1ug pUC 19 for 1hour at 37C.

Unit Definition:
One unit hydrolyses 1ug of nucleic acid per minute at 37C, pH 4.6.
Vogt, G. M., Eur. J. Biochem., 33:192 (1973).
Roberts, T. M., et al., Proc. Natl. Acad. Sci, 76:160 (1979).
Berk, A. J., and Sharp, P. A., Proc. Natl. Acad. Sci, 75 (1979)

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.