Technical Data
S100 Calcium Binding Protein A4 (S100A4)
S100 belongs to the family of calcium binding proteins such as calmodulin and troponin C. S100A is composed of an alpha and beta chain whereas S100B is composed of two beta chains. S100 protein is also expressed in the antigen presenting cells such as the Langerhans cells in skin and interdigitating reticulum cells in the paracortex of lymph nodes. S100A4 protein belongs to the S100 subfamily, now designated S100A1-S100A13.

Suitable for use in Immunohistochemistry. Other applications not tested.

Recommended Dilutions:
Immunohistochemistry (Formalin/paraffin): 1:100-1:200 for 30 minutes at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 minutes followed by cooling at RT for 20 minutes.
Optimal dilution to be determined by researcher.

Positive Control:

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
250ul-20CBlue IceHumanRabbit
Not Determined
Purified recombinant human S100A4 monomer expressed in E. coli. Cellular Localization: Cytoplasmic, membrane and nuclear.
Supplied as a liquid in 10mM PBS, pH 7.4, 0.2% BSA, 0.09% sodium azide.
Recognizes human S100A4, MW ~10-12kD. Species Crossreactivity: Mouse, Rat, Bovine, Equine, Feline and Canine.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Schafer, B.W., et al., Trends Biochem. Sci. 21: 134-140 (1996). 2. Ilg, E.C., et al., Intl. J. Cancer 68: 325-332 (1996).