Technical Data
S100 Calcium Binding Protein A4 (S100A4)
S100 calcium binding protein A4 (S100A4) is a member of the S100 family of calcium-binding proteins that contain two Ca(2+)-binding sites including a canonical EF-hand motif. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells. S100A4 interacts with cytoskeletal proteins and enhances metastasis of several types of cancer cells. It is secreted by unknown mechanisms, thus, paracrinely stimulating a variety of cellular responses, including angiogenesis and neuronal growth. S100A4 has been shown to be a prognostic marker in a number of human cancers, including esophageal-squamous cancers, non-small lung cancers, primary gastric cancers, malignant melanomas, prostate cancers, bladder cancers, and pancreatic carcinomas. The universality of S100A4 expression in a variety of cancers illustrates the potential use of S100A4 as a marker for tumor metastasis and disease progression.

Suitable for use in ELISA and Flow Cytometry. Other applications not tested.

Recommended Dilution:
ELISA: 1:200-1:400
Flow Cytometry: 1.2ug/10e6 cells
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
50ug-20CBlue IceHumanMouse
Genetic immunization with cDNA encoding S100 A4.
Purified by Protein G affinity chromatography.
Supplied as a liquid in PBS, pH 7.2.
Recognizes human S100 Calcium Binding Protein A4. Recognizes the complete native protein expressed on transfected mammalian cells.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.