		Technical Data
S0051-84	S100 Calcium Binding Protein A4 (S100A4)

Description:
S100 belongs to the family of calcium binding proteins such as calmodulin and troponin C. S100A is composed of an alpha and beta chain whereas S100B is composed of two beta chains. S100 protein is also expressed in the antigen presenting cells such as the Langerhans cells in skin and interdigitating reticulum cells in the paracortex of lymph nodes.

Applications:
Suitable for use in ELISA and Western Blot. Other applications not tested.

Recommended Dilution:
ELISA: 0.5-1ug/ml
Western Blot: 1ug/ml
Optimal dilutions to be determined by the researcher.

Storage and Stability:
Lyophilized powder may be stored at -20°C for short-term only. Reconstitute with sterile 40-50% glycerol, aliquot and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Type	Isotype	Clone	Grade
Pab	IgG		Affinity Purified

Size		Storage	Shipping	Source	Host
100ug		-20°C	Blue Ice	Mouse	Sheep

Concentration:

As reported

Immunogen:

E. coli-derived recombinant mouse S100A4.

Purity:

Purified by immunoaffinity chromatography.

Form

Supplied as a lyophilized powder in PBS, 5% trehalose. Reconstitute with sterile PBS.

Specificity:

Recognizes mouse S100A4. Less than 1% cross-reactivity with S100A8, A9 and A10. Species Crossreactivity: human.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.