Technical Data
S0051-86A
S100 Calcium Binding Protein A9 (S100A9, 60B8AG, Calgranulin B, CAGB, Calprotectin L1H Subunit, CFAG, CGLB, L1AG, Leukocyte L1 Complex Heavy Chain, LIAG, MAC387, MIF, Migration Inhibitory Factor-related Protein 14, MIF Related Protein 14, MRP14, MRP-14, N
Description:
S100 Calcium Binding Protein A9, also known as Calgranulin B, belongs to the S100 family containing EF-hand type Ca2+-binding proteins. It is involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation, and associated with the disease cystic fibrosis. S100A9 has been implicated in the abnormal differentiation of myeloid cells in the stroma of cancer. This protein may function in the inhibition of casein kinase.

Applications:
Suitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Immunohistochemistry (paraffin-embedded): Pretreat with trypsin.
Immunohistochemistry: Frozen
Optimal dilutions to be determined by the researcher.

Hybridoma:
NS-1 mouse myeloma cells with spleen cells from Balb/c mice.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgG147-8D3Purified
SizeStorageShippingSourceHost
100ug-20CBlue IceHumanMouse
Concentration:
~0.1mg/ml
Immunogen:
Human peripheral blood monocyte components.
Purity:
Purified
Form
Supplied as a liquid in PBS, 0.05% sodium azide.
Specificity:
Recognizes S100 Calcium Binding Protein A9 at 14kD in human monocyte and granulocyte lysates. Recognizes macrophages and leukocyte L1.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.