Technical Data
S0051-88K
S100A12 (Protein S100-A12, CGRP, Calcium-binding Protein in Amniotic Fluid 1, CAAF1, Calgranulin-C, CAGC, Extracellular Newly Identified RAGE-binding Protein, EN-RAGE, Neutrophil S100 Protein, S100 Calcium-binding Protein A12, p6) (PE)
Description:
EN-RAGE, also known as S100A12 and Calgranulin C, is a 10kD secreted molecule that belongs to the S100 family of calcium binding proteins. EN-RAGE contains two EF hand domains and forms dimers and hexamers in solution. EN-RAGE is overexpressed at sites of inflammation and interacts with RAGE on endothelial cells, lymphocytes, and monocytes to promote inflammatory responses. Human EN-RAGE shares 66% and 70% aa sequence identity with bovine and porcine EN-RAGE, respectively; mouse and rat orthologs have not been identified.

Applications:
Suitable for use in Flow Cytometry. Other applications not tested.

Recommended Dilution:
Optimal dilutions to be determined by the researcher.

Excitation: 488nm

Emission: 565-605nm

Storage and Stability:
May be stored at 4C before opening. DO NOT FREEZE! Stable at 4C as an undiluted liquid. Dilute only prior to immediate use. Stable for 12 months at 4C. Freezing R-Phycoerythrin (PE) conjugates will result in a substantial loss of activity. PE conjugates are sensitive to light.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
100Tests4C Do Not FreezeBlue IceHumanGoat
Concentration:
Not determined
Immunogen:
Recombinant corresponding to aa1-92 from human EN-RAGE expressed in E.coli.
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a liquid in saline, 0.5% BSA, 0.1% sodium azide. Labeled with R-Phycoerythrin (PE).
Specificity:
Recognizes human EN-RAGE.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.