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Technical Data |
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S0051-88M |
S100 Calcium Binding Protein A12 (S100A12, CAAF1, Calcium-binding Protein in Amniotic Fluid, Calgranulin-C, CAGC, Calgranulin Related Protein, CGRP, EN-RAGE, ENRAGE, Extracellular Newly Identified RAGE-binding Protein, Neutrophil S100 Protein, p6) |
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Description: S100 family proteins are acidic low molecular weight proteins containing 2 EF-hand calcium binding domains. They are characterized by their cell-type specific expression patterns. S100A12 is a member of the calgranulin sub-family. Calgranulins are S100 proteins that are expressed by neutrophils and also found in infiltrating monocytes and granulocytes during chronic inflammation. S100A12 is expressed in neutrophils and monocytes. It is highly abundant in resting neutrophils, comprising 5% of total cytostolic protein. S100A12 is a ligand for RAGE. Interaction with RAGE on mononuclear phagocytes, lymphocytes and endothelium triggers cellular activation. Nuclear factor kappa B, a key transcription factor for inflammatory events, is activated by S100A12/RAGE. S100A12 has also been shown to have anti-microbial properties. Applications: Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested. Recommended Dilutions: Western Blot: 1:200-1:1000 Immunohistochemistry (frozen/ paraffin): 1:100-1:500 Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. |
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