Technical Data
S0052-03B
S100 Protein (Bpb, NEF, S100 alpha, S100A, S100 beta, S100beta, S100B, S100 Calcium Binding Protein A1, S100a1, S100 Calcium Binding Protein B, S100 Calcium Binding Protein beta Neural, S100 Protein alpha Chain, S-100 Protein alpha Chain, S100 Protein alp
Description:
S100 belongs to the family of calcium binding proteins such as calmodulin and troponin C. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21. S100A is composed of an alpha and beta chain whereas S100B is composed of two beta chains. S100 protein may function in stimulation of Ca2+ induced Ca2+ release, inhibition of microtubule assembly, and inhibition of protein kinase C mediated phosphorylation. Reduced expression of this protein has been implicated in cardiomyopathies. Immunoreactive S100 protein localizes in the cytoplasm and nuclei of astrocytes, Schwann's cells, ependymomas and astrogliomas. Ganglion cells do not stain for S100 protein. S100 can be detected in almost all benign naevi and malignant melanocytic tumors of the skin, and in Langerhans cells in the skin.

Applications:
Suitable for use in ELISA and Western Blot. Other applications not tested.

Recommended Dilution:
Optimal dilutions to be determined by the researcher.

Matched Pair:
Capture: S0052-03B
Detection: S0052-11N

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgG19E653Affinity Purified
SizeStorageShippingSourceHost
200ug-20CBlue IceHumanMouse
Concentration:
~8mg/ml
Immunogen:
Full length human S100 protein. Cellular Localization: Cytoplasmic.
Purity:
Purified by Protein A affinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.4, 0.1% sodium azide. Note: This antibody is sensitive to EDTA or other bivalent-binding agents. Better performance can be obtained in presence of 5 mM CaCl2 in the assay buffers.
Specificity:
Recognizes human S-100 beta beta and S100 alpha beta at ~21kD. Does not recognize the alpha subunit alone.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.