Technical Data
S100 Protein
Calbindins are Ca-binding proteins belonging to the troponin C superfamily. There are two types of CaBPs: the "trigger"- and the "buffer"-CaBPs. The conformation of "trigger" type CaBPs changes upon Ca2+ binding and exposes regions on protein that interact with target molecules, thus altering their activity. The buffer-type CABP are thought to control the intracellular calcium concentration. S100 protein or Ca-binding protein A1/S100A1 is also a member of low mol wt, cytoplasmic, calcium binding troponin family. It is produced in the brain, muscle, and heart and a wide variety of normal and tumor cells. S100 has two subunits: S100-alpha (mouse/rat/human 94 aa, chromosome 1q21) and S100-beta (mouse, rat/human 92 aa chromosome 21q22.2-q22.3) that forms either homodimer (alpha-alpha known as S-100a(0) or beta-beta known as S-100b) and heterodimers (known as S-100a) of ~21kD. S100 alpha and beta chains show ~58% sequence identity. 9 different genes for S100 have been assigned on chromosome 21 namely S100A1-S1009. S-100 contains 2 EF-hand domains. S100A1 improves cardiac contractile performance both by regulating sarcoplasmic reticulum Ca ion handling myofbirilar Ca-ion responsiveness. Leakage of S100 protein by glial cells into the extracellualr matrix, CSF and serum are useful in monitoring traumatic brain injury.

Suitable for use in Immunofluorescence, ELISA, Western Blot and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Western Blot: 1:2000-5000 ECL. S100 protein (bovine and human) is ~21kD.
ELISA: 1:10,000-1:100,000
Immunohistochemistry/Immunofluorescence: 1:50-1:100 in indirect staining of formalin-fixed, paraffin embedded tissue section.
Optimal dilutions to be determined by the researcher.

Positive Control for Western Blot (Human): S0052-10B

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
PabIgGAffinity Purified
50ug-20CBlue IceBovineRabbit
Purified bovine S100 protein (containing both alpha and beta chains).
Purified by immunoaffinity chromatography.
Supplied as a liquid in PBS, pH 7.5, 0.1% BSA.
Recognizes bovine S100 Protein. No significant reactivity is seen with other CABPs. Species Crossreactivity: rat and human S100 intact or alpha and beta chains in both neural and non-neural tissues.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Engelkamp D et al (1993) PNAS 90, 6547-6551; 2. Herrera GA et al (1988) Am. J. Pathol. 89, 168-176; 3. Isobe T et al (1978) J. Neurochem. 30, 921-923; 4. Morri K et al (1991) BBRC 175, 185-191; 5. Most P et al (2001) PNAS 98, 13889-13894; 6. Schafer BW et al (1995) Genomics 25, 638-643