Technical Data
Media
Sabouraud Dextrose Agar w/Chloramphenicol (Powder)
S0053-15
Microbiological Grade
Storage RT/4C    Shipping RT
Components: g/Liter
Casein Peptone5.0
Dextrose40.0
Meat Peptone5.0
Agar15.0
Chloramphenicol0.05
Total: 65.0g/liter
Application:
Selective for fungi isolation with inhibition of many bacteria.

Appearance:
Beige, homogeneous free-flowing powder

Solubility: Visually the prepared medium is clear to trace hazy and light amber in color.

pH (6.5%): ~5.6

Microbiological Tests:
Aspergillus niger ATCC 16404:Good
Candida albicans ATCC 10231: Good
Escherichia coli ATCC 25922: Inhibited
Saccharomyces cerevisiae ATCC 18790: Good


Directions per Liter: Dissolve 65 grams in 800-900ml of ddH2O stirring gently with heating until completely solubilized. Adjust pH of the medium to the desired level. Add additional water to bring the solution to1L. If required, add sodium bicarbonate. Dispense into appropriate containers, loosen caps and autoclave for 15 minutes at 121C (15psi).

Storage and Stability: Store powdered media at RT. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept at 4C and used within a short period of time.


Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.