Technical Data
Media
Sabouraud Dextrose Agar w/Lecithin and Tween 80 (Powder)
S0053-20
Microbiological Grade
Storage RT/4°C    Shipping RT
Components: (g/liter)
Casein Digest Peptone5.0
Dextrose40.0
Peptic Digest of Animal Tissue5.0
Lecithin0.7
Tween 805.0
Agar15.0
Total:71.0g/liter
Medium employed for surface sampling techniques to enumerate yeasts and molds, inhibits most gram positive bacteria.

Appearance:
Light beige, homogeneous, free flowing with slight lumps.

Solubility:
Light amber, clear to trace hazy

pH:
5.6 ± 0.2

Growth*:
Aspergillus niger ATCC 16404
Candida albicans ATCC 10231
Microsporum audounii ATCC 42588
Microsporum canis ATCC 36299
Penicillum roquefortii ATCC10110
Trichophyton mentagrophytes ATCC 9533

* These results were obtained from type cultures after incubation at a temperature of 25°C and observed after 3-5 days.

Directions:
Mix 71.0g per liter of ddH2O until evenly dispersed. Heat with repeated stirring and boil for one minute to dissolve completely. Distribute and autoclave at 121.0°C for 15 minutes.

Storage and Stability:
Store powdered media at RT. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept refrigerated and used within a short period of time.


Directions per Liter: Mix 71.0g per liter of ddH2O until evenly dispersed. Heat with repeated stirring and boil for one minute to dissolve completely. Dispense into appropriate containers, loosen caps and autoclave for 15 minutes at 121ºC (15psi).

Storage and Stability: Store powdered media at RT. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept at 4°C and used within a short period of time.

Appearance: Light beige, homogeneous, free flowing with slight lumps.

Solubility: Light amber, clear to trace hazy

pH: 5.6 ± 0.2


Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.