Technical Data
S0059-07B
Salmon (Salmonid, a-SIg) (HRP)
Description:
Used for the detection of salmonid parasites (bacteria or viruses), indirectly by detecting parasite specific immunoglobulins from Salmo, Oncorhynchus and Salvelinus salmon. Also used for the detection of unknown parasites by monitoring the level of serum immunoglobulins in salmonid stock and responses to specific parasites, or to assess the efficacy of salmonid vaccines.

Applications:
Suitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested.

Recommended Dilutions:
ELISA: 1:500-1:1000
Western Blot: 1:400-1:800
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Labeled with horseradish peroxidase (HRP). Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. HRP conjugates are sensitive to light.
TypeIsotypeCloneGrade
MabIgG2a2J3 (5-F12)Affinity Purified
SizeStorageShippingSourceHost
50ug-20CBlue IceMouse
Concentration:
~1mg/ml
Immunogen:
Rainbow Trout and Atlantic Salmon heavy and light chain immunoglobulins.
Purity:
Purified by Protein G affinity chromatography
Form
Supplied as a liquid in PBS, pH 7.2, 10mg/ml BSA, 0.01% gentamicin sulfate. Labeled with horseradish peroxidase (HRP).
Specificity:
Recognizes Salmo, Oncorhynchus and Salvelinus serum immunoglobulins. Reacts with Atlantic Salmon, Brown Trout, Chinook Salmon, Chum Salmon, Coho Salmon, Sockeye Salmon, Rainbow Trout, Arctic Char, Brook Trout. Does not recognize China Perch or Zebra Fish.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.