Description:
Applications:
Suitable for use in ELISA and Western Blot. Other applications not tested.
Recommended Dilution:
ELISA: 1:50-1:500
Optimal dilutions to be determined by the researcher.
Affinity Constant:
3.3x10e10L/M
Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
|
| Type | Isotype | Clone | Grade |
|---|
| Mab | IgG1 | 8.F.253 | Affinity Purified |
|
| Size | | Storage | Shipping | Source | Host |
|---|
| 200ug | | 4°C (-20°C Glycerol) | Blue Ice | | Mouse |
|
| Concentration: |
| ~3mg/ml |
| Immunogen: |
| Salmonella typhimurium mixture of ~90% of the “O” antigens. Each serotype was grown overnight and harvested. The suspension was washed twice by centrifugation and resuspended in a physiological saline buffer. |
| Purity: |
| Purified by Protein A affinity chromatography. |
| Form |
| Supplied as a liquid in PBS, pH 7.2, 0.05% sodium azide, before the addition of glycerol to 40%. |
| Specificity: |
| Recognizes LPS of Salmonella typhimurium. Recognizes the “O” somatic antigens. Does not recognize the“H” flagella antigens. |
|
| Intended for research use only. Not for use in human, therapeutic, or diagnostic applications. |
|
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