Technical Data
S0060-10C
Salmonella
Description:
The genus Salmonella is a member of the family Enterobacteriaceae. The genus is composed of Gram negative bacilli that are facultative and flagellated (motile). Salmonellae possess 3 major surface antigens; the H or flagellar antigen (phase 1 and 2), the O or somatic antigen (part of the LPS moiety) and the Vi or capsular antigen (referred to as K in other Enterobacteriaceae). Salmonellae also possess the LPS endotoxin characteristic of Gram negative bacteria. This LPS is composed of an O polysaccharide (O antigen) an R core and the endotoxic inner Lipid A. Endotoxins evoke fever and can activate complement, kinin and clotting factors.

Hybridoma:
X63-Ag8.653 myeloma cells with spleen cells from Balb/c mice.

Applications:
Suitable for use in ELISA. Other applications not tested.

Recommended Dilution:
ELISA: 1:10
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
MabIgG2b9L639Supernatant
SizeStorageShippingSourceHost
2ml-20°CBlue IceMouse
Concentration:
Not determined
Immunogen:
Flagellar preparation from S. hadar.
Purity:
Supernatant
Form
Supplied as a liquid, 0.09% sodium azide.
Specificity:
Recognizes S. hadar-100%; S. typhimurium (phase 1)- 48%; S. typhimurium (phase 2)-137%; S. enteritidis-47%; S. virchow-40%; S. bredeney-55%; S. weltevreden-80%; S. infantis-91%; S. agona-31%.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Wyatt, G.M., et al., (1993), “Further studies on the feasibility of one-day. Salmonela detection by enzyme-linked immunosorbent assay”, Applied and Environmental Microbiology, 58: 183-1390.