Technical Data
S0060-13
Salmonella hadar
Description:
Applications:
Suitable for use in ELISA. Other applications have not been tested.

Recommended Dilution:
ELISA: 1:20
Optimal working dilutions to be determined by researcher.

Fusion Partners:
Spleen cells from immunized Balb/c mice were fused with cells of the X63-Ag8-653 myeloma cell line.

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
MabIgG2b3H2879Supernatant
SizeStorageShippingSourceHost
2ml4C (-20C Glycerol)Blue IceMouse
Concentration:
Not determined
Immunogen:
Flagellar preparation from S. hadar.
Purity:
Tissue culture supernatant.
Form
Supplied as a liquid with 0.09% sodium azide.
Specificity:
Recognizes the following:S.hadar-100%; S. typhimurium (phase 1)-48%; S. typhimurium (phase 2)-137%; S. enteritidis-47%; S. virchow-40%; S. bredeney-55%; S. weltevreden-80%; S. infantis-91%; S. agona-31%.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Wyatt, G.M. et al. (1993). Further stdies on the feasibility of one-day. Salmonelaa detection by enzyme-linked immunosorbent assay. Applied and Environmental Microbiology. 59: 183-1390.