Technical Data
S0060-18A
Salmonella paratyphi A
Description:
S. enterica has an extraordinarily large number of serovars or strains—up to 2000 have been described.[2] Salmonella enterica Serovar Typhi (historically elevated to species status as S. typhi) is the disease agent in typhoid fever. The genome sequences of Serovar Typhi has been established.[3] Salmonella enterica Serovar Typhimurium (also known as S. typhimurium) can lead to a form of human gastroenteritis sometimes referred to as salmonellosis. The genome sequences of serovar Typhimurium LT2[4] have been established. Also an analysis of the proteome of Typhimurium LT2 under differing environmental conditions has been performed [5]. Salmonella enterica Serovar Paratyphi A has been identified.[6] It is associated with paratyphoid fever. It is sometimes known as Salmonella paratyphi.

Most cases of salmonellosis are caused by food infected with S. enterica, which often infects cattle and poultry, though also other animals such as domestic cats and hamsters[7] have also been shown to be sources of infection to humans. However, investigations of vacuum cleaner bags have shown that households can act as a reservoir of the bacterium; this is more likely if the household has contact with an infection source, for example members working with cattle or in a veterinary clinic. Raw chicken and goose eggs can harbor S. enterica, initially in the egg whites, although most eggs are not infected. As the egg ages at room temperature, the yolk membrane begins to break down and S. enterica can spread into the yolk. Refrigeration and freezing do not kill all the bacteria, but substantially slow or halt their growth. Pasteurizing and food irradiation are used to kill Salmonella for commercially-produced foodstuffs containing raw eggs such as ice cream. Foods prepared in the home from raw eggs such as mayonnaise, cakes and cookies can spread salmonella if not properly cooked before consumption.

Secreted proteins are of major importance for the pathogenesis of infectious diseases caused by Salmonella enterica. A remarkable large number of fimbrial and non-fimbrial adhesins are present in Salmonella and mediate biofilm formation and contact to host cells. Secreted proteins are also involved in host cell invasion and intracellular proliferation, two hallmarks of Salmonella pathogenesis.[8]

Applications:
Suitable for use in ELISA and Western Blot. Other applications not tested.

Recommended Dilution:
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
MabIgG19E662Affinity Purified
SizeStorageShippingSourceHost
100ug-20°CBlue IceMouse
Concentration:
As reported
Immunogen:
Heat inactivated bacterial cells of Salmonella paratyphi A.
Purity:
Purified by Protein G affinity chromatography.
Form
PBS, pH 7.4 with 0.1% sodium azide
Specificity:
This antibody reacts with group A 0-2 antigen. Binding constants (Ka, Me-1) of S0060-18A LPS by the Beatty (et al) method: LPS A = 2.0 x 10^7 LPS B, D, E = negligible Species Crossreactivity: This antibody detects Salmonella paratyphi A. It does not react with other Salmonella, E. coli, or Klebsiella.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
General References:
1. Giannella RA (1996). Salmonella. In: Baron's Medical Microbiology (Barron S et al., eds.) (4th ed.). Univ of Texas Medical Branch. (via NCBI Bookshelf) ISBN 0-9631172-1-1.
2. Ryan KJ; Ray CG (editors) (2004). Sherris Medical Microbiology (4th ed.). McGraw Hill. ISBN 0-8385-8529-9.
3. Parkhill J et al. (2001). "Complete genome sequence of a multiple drug resistant Salmonella enterica serovar Typhi CT18". Nature 413 (6858): 848–52. doi:10.1038/35101607. PMID 11677608 doi:10.1038/35101607.
4. McClelland M et al. (2001). "Complete genome sequence of Salmonella enterica serovar Typhimurium LT2". Nature 413 (6858): 852–6. doi:10.1038/35101614. PMID 11677609 doi:10.1038/35101614.
5. Adkins JN et al. (2006). "Analysis of the Salmonella typhimurium Proteome through Environmental Response toward Infectious Conditions". Molecular and Cellular Proteomics 5: 1450–1461. doi:10.1074/mcp.M600139-MCP200. PMID 16684765.
6. Huang H, Li J, Yang XL, et al. (January 2009). "Sequence Analysis of the Plasmid pGY1 Harbored in Salmonella enterica Serovar Paratyphi A". Biochem. Genet. 47: 191. doi:10.1007/s10528-008-9216-0. PMID 19169860.
7. Swanson SJ, Snider C, Braden CR, et al. (2007). "Multidrug-resistant Salmonella enterica serotype Typhimurium associated with pet rodents". New England Journal of Medicine 356 (1): 21–28. doi:10.1056/NEJMoa060465. PMID 17202452.
8. Hensel M (2009). "Secreted Proteins and Virulence in Salmonella enterica". Bacterial Secreted Proteins: Secretory Mechanisms and Role in Pathogenesis. Caister Academic Press. ISBN 978-1-904455-42-4.

http://en.wikipedia.org/wiki/Salmonella_typhimurium