Technical Data
S0060-18B
Salmonella paratyphi A
Description:
The O antigen is a polysaccharide that extends from the core oligosaccharide, which itself is attached to lipid A. These three components together form the lipopolysaccharide (LPS), a major component of the outer membrane of Gram-negative bacteria. The LPS is an endotoxin and induces a strong immune response from the host. Its mutation or removal results in the death of the bacterial cell and as such is a prime target for antimicrobial drugs. The O antigen is the outermost component of the LPS and functions as a receptor for bacteriophages. It is also important in the immune response of the host. Considerable diversity is seen within Salmonella O antigens.

Salmonella enterica, serovar paratyphi A belongs to serogroup A of Salmonella and causes paratyphoid fever, which is similar to typhoid fever but more benign.

Applications:
Suitable for use in ELISA, Immunoblot, and Immunofluorescence. Other applications not tested.

Recommended Dilution:
Optimal dilutions to be determined by the researcher.

Hybridoma:
Sp2/0 myeloma cells with spleen cells from Balb/c mice.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgG110F421Affinity Purified
SizeStorageShippingSourceHost
200ug-20CBlue IceMouse
Concentration:
~1mg/ml
Immunogen:
Heat inactivated cells of Salmonella paratyphi A.
Purity:
Purified by Protein G affinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.4, 0.09% sodium azide.
Specificity:
Recognizes the O antigen of Salmonella enterica, serovar paratyphi A (also known as S. paratyphi), and it is specific for the 0-2 antigenic determinant of Salmonella serogroup A and does not cross-react with the LPS of serogroups B, D or E of Salmonella spp.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.