Technical Data
S0060-23A
Salmonella sp.
Description:
Salmonella is a genus of the family Enterobacteriaceae populated by a variety of Gram negative rod-shaped bacteria, many of which are pathogenic and cause a range of diseases in humans. Salmonellae possess 3 major surface antigens; the H or flagellar antigen (phase 1 and 2), the O or somatic antigen (part of the LPS moiety) and the Vi or capsular antigen (referred to as K in other Enterobacteriaceae). Salmonellae also possess the LPS endotoxin characteristic of Gram negative bacteria. This LPS is composed of an O polysaccharide (O antigen) an R core and the endotoxic inner Lipid A.

Applications:
Suitable for use in ELISA. Other applications not tested.

Recommended Dilution:
ELISA: 1:20-1:200
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgG110F420Affinity Purified
SizeStorageShippingSourceHost
100ug-20CBlue IceMouse
Concentration:
0.1mg/ml
Immunogen:
Native
Purity:
Purified by Protein A affinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.2, 0.1% sodium azide.
Specificity:
Recognizes the flagellum protein present in most Salmonella species and is broadly reactive in ELISA. Does not detect E. coli and other intestinal bacteria.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. McSorley S. J. et al. (2000) Characterization of CD4+ T cell responses during natural infection with Salmonella typhimurium. J. Immunol. 164: 986-993.