Technical Data
Salmonella-Shigella Agar (Powder)
Microbiological Grade
Storage RT    Shipping RT
Formula g/l:
Pancreatic Digest of Tissue2.5
Beef Extract5.0
Bile Salts Mixture8.5
Peptic Digest of Animal Tissue2.5
Sodium Citrate8.5
Sodium Thiosulfate8.5
Ferric Citrate1.0
Neutral Red0.025
Brilliant Green0.00033
Selective and differential medium for isolation of Salmonella and some shigella strains from clinical and food materials.

Description: Salmonella Shigella Agar is a selective differential agar medium used for isolation of pathogenic Enterobacteriaceae, specifically Salmonella sp. and Shigella sp. from foods, other sanitary materials and clinical specimens. Early studies, indicated that SS Agar was superior to other media in the isolation of Salmonella and Shigella from specimens. Later investigators, determined that SS Agar was inferior to Hektoen Enteric Agar and XLD Agar in the recovery of Shigella. Inhibitory action on coliforms and Gram-positive bacteria is obtained by the bile salts mixture and brilliant green in the medium. Sodium citrate inhibits Gram-positive bacteria. Neutral red is a pH indicator and those bacteria which ferment lactose produce pink colonies. Some Salmonella and Proteus sp. produce black-centered (precipitated ferrous sulfate) colonies as a result

Directions per Liter: Dissolve 60 grams per liter of distilled/deionized (DDI) water. Heat with repeated stirring and boil for one minute to dissolve completely. DO NOT AUTOCLAVE

Storage and Stability: Store powdered media at RT. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept at 4C and used within a short period of time.

Appearance: Light pinkish-beige, homogenous, free flowing Solubility

Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.