Technical Data
S0060-26E
Salmonella species (A,B,C,D,E,F and G groups)
Description:
The genus Salmonella is a member of the family Enterobacteriaceae. The genus is composed of Gram negative bacilli that are facultative and flagellated (motile). Salmonellae possess 3 major surface antigens; the H or flagellar antigen (phase 1 and 2), the O or somatic antigen (part of the LPS moiety) and the Vi or capsular antigen (referred to as K in other Enterobacteriaceae). Salmonellae also possess the LPS endotoxin characteristic of Gram negative bacteria. This LPS is composed of an O polysaccharide (O antigen) an R core and the endotoxic inner Lipid A. Endotoxins evoke fever and can activate complement, kinin and clotting factors.

Applications:
Suitable for use in ELISA and Immunofluorescence. Other applications have not been tested.

Recommended Dilutions:
ELISA: LPS extraction recommended
Optimal dilutions to be determined by the researcher.

Recommended Pairs:
Capture Detection
S0060-26F S0060-26E
S0060-26D S0060-26E

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgG38J140Affinity Purified
SizeStorageShippingSourceHost
100ug-20°CBlue IceMouse
Concentration:
~0.1mg/ml
Immunogen:
Mixture of 4 Salmonella serotypes (whole organisms)
Purity:
Purified by Protein A chromatography ( >90%)
Form
Supplied as a liquid in PBS, pH 7.2, 0.1% sodium azide. No stabilizing proteins.
Specificity:
Recognizes common LPS core of Salmonella. Positive for all Salmonella O-serogroups tested: A, B, C1, C2, D, E1, E3, E4, F, G1 and G2. Negative for E. coli (mix), Klebsiella, Citrobacter, Pseudomonas, Yersinia, Shigella, Proteus and Legionella.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.