Technical Data
S0060-29C
Salmonella sp. (FITC)
Description:
Salmonella is a genus of the family Enterobacteriaceae populated by a variety of Gram negative rod-shaped bacteria, many of which are pathogenic and cause a range of diseases in humans. Salmonellae possess 3 major surface antigens; the H or flagellar antigen (phase 1 and 2), the O or somatic antigen (part of the LPS moiety) and the Vi or capsular antigen (referred to as K in other Enterobacteriaceae). Salmonellae also possess the LPS endotoxin characteristic of Gram negative bacteria. This LPS is composed of an O polysaccharide (O antigen) an R core and the endotoxic inner Lipid A.

Applications:
Suitable for use in Immunofluorescence. Other applications not tested.

Recommended Dilution:
Immunofluorescence: 1:10-1:50. Direct FA staining of target antigens in a permissive tissue culture system. Acetone fixation of the antigen source is recommended prior to staining.
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20C. Aliquots are stable for at least 6 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabIgGHighly Purified
SizeStorageShippingSourceHost
1ml-20CBlue IceRabbit
Concentration:
~4-5mg/ml
Immunogen:
Mixture of S. enteriditis, S. typhimurium & S. heidelburg
Purity:
Purified IgG fraction to a purity of > 95%.
Form
Supplied as a liquid in PBS, pH 7.2, BSA (10mg/ml), 0.1% sodium azide. Conjugated to FITC.
Specificity:
Polyvalent for Salmonella "O" & "H" antigens. Antiserum is unabsorbed and does crossreact with related EnterobacteriaceaeI. mmunocaptures Salmonellae
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.