Technical Data
S0060-32B
Salmonella typhimurium
Description:
The genus Salmonella is a member of the family Enterobacteriaceae. The genus is composed of Gram-negative bacilli that are facultative and flagellated (motile).

Salmonellae possess 3 major antigens; the "H" or flagellar antigen (phase 1 & 2), the "O" or somatic antigen (part of the LPS moiety) and the "Vi" or capsular antigen (referred to as "K" in other Enterobacteriaceae). Salmonellae also possess the LPS endotoxin characteristic of Gram-negative bacteria. This LPS is composed of an "O" polysaccharide ("O" antigen) an "R" core and the endotoxic inner "Lipid A". Endotoxins evoke fever and can activate complement, kinin and clotting factors.

Until recently the most common cause of food poisoning by Salmonella species was due to S. Typhimurium. As its name suggests, it causes a typhoid-like disease in mice. In humans S. Typhimurium does not cause as severe disease as S. Typhi, and is not normally fatal. The disease is characterized by diarrhea, abdominal cramps, vomiting and nausea, and generally lasts up to 7 days.

Hybridoma:
X63-Ag8.653 myeloma cells with spleen cells from Balb/c mice.

Applications:
Suitable for use in ELISA and Flow Cytometry. Other applications not tested.

Recommended Dilution:
ELISA: 1:20
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
MabIgM9L640Supernatant
SizeStorageShippingSourceHost
2ml-20°CBlue IceMouse
Concentration:
Not determined
Immunogen:
Flagellar preparation of S. typhimurium (Phase 1).
Purity:
Supernatant
Form
Supplied as a liquid, 0.09% sodium azide.
Specificity:
Recognizes S. typhimurium (phase 1)-100%; S. typhimurium (phase 2)-65%; S. agena-7%.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Lee, H.A., et al., (1990), “Enzyme-linked immunosorbent assay for Salmonella typhimurium in food: Feasibility of 1-day Salmonella detection”, Applied Environmental Microbiology, 56: 1541-1546.