Technical Data
S0060-40C
Salmonella enteritidis LPS
Description:
The genus Salmonella is a member of the family Enterobacteriaceae. The genus is composed of Gram negative bacilli that are facultative and flagellated (motile). Salmonellae possess 3 major surface antigens; the H or flagellar antigen (phase 1 and 2), the O or somatic antigen (part of the LPS moiety) and the Vi or capsular antigen (referred to as K in other Enterobacteriaceae). Salmonellae also possess the LPS endotoxin characteristic of Gram negative bacteria. This LPS is composed of an O polysaccharide (O antigen) an R core and the endotoxic inner Lipid A. Endotoxins evoke fever and can activate complement, kinin and clotting factors.

Applications:
Suitable for use in ELISA and Western Blot. Other applications not tested.

Recommended Dilutions:
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgG110F419Affinity Purified
SizeStorageShippingSourceHost
200ug-20CBlue IceMouse
Concentration:
~1mg/ml
Immunogen:
Heat inactivated cells from Salmonella (LPS groups A, B & D).
Purity:
Purified by Protein G affinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Specificity:
Recognizes a common antigenic determinant that is found in the LPS of Salmonella enteriditis (Group D), Salmonella typhimurium (Group B) and Salmonella paratyphi (Group A). Thought to recognize the '0-12' antigen [2-D-mannose (1-4) I-ramnose (1-3)-D galactose] of Salmonella LPS. A similar sequence is found in Group E, which may account for the weak cross reactivity observed with bacteria of this group.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.