Technical Data
S0060-53
Salmonella sp.
Description:
The genus Salmonella is a member of the family Enterobacteriaceae. The genus is composed of Gram negative bacilli that are facultative and flagellated (motile). Salmonellae possess 3 major surface antigens; the H or flagellar antigen (phase 1 and 2), the O or somatic antigen (part of the LPS moiety) and the Vi or capsular antigen (referred to as K in other Enterobacteriaceae). Salmonellae also possess the LPS endotoxin characteristic of Gram negative bacteria. This LPS is composed of an O polysaccharide (O antigen) an R core and the endotoxic inner Lipid A. Endotoxins evoke fever and can activate complement, kinin and clotting factors.

Applications:
Suitable for use in Immunofluorescence and ELISA. Other applications not tested.

Recommended Dilutions:
Immunofluorescence: 1:10-1:50
ELISA: 1:20-1:200
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgG2a5E433Affinity Purified
SizeStorageShippingSourceHost
1ml-20CBlue IceMouse
Concentration:
~0.1mg/ml
Immunogen:
Salmonella
Purity:
Purified by Protein A affinity chromatography
Form
Supplied as a liquid in PBS, pH 7.2, 0.1% sodium azide. No stabilizing proteins have been added.
Specificity:
Recognizes common LPS core of all Salmonellae tested. Positive for all Salmonella O-serogroups tested: A, B, C1, C2, D, E1, E3, E4, F, G1 and G2. Negative for E. coli (mix), Klebsiella, Citrobacter, Pseudomonas, Yersinia, Shigella, Proteus and Legionella
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.