Technical Data
S0060-53A
Salmonella sp. (FITC)
Description:
The genus Salmonella is a member of the family Enterobacteriaceae. The genus is composed of Gram negative bacilli that are facultative and flagellated (motile). Salmonellae possess 3 major surface antigens; the H or flagellar antigen (phase 1 and 2), the O or somatic antigen (part of the LPS moiety) and the Vi or capsular antigen (referred to as K in other Enterobacteriaceae). Salmonellae also possess the LPS endotoxin characteristic of Gram negative bacteria. This LPS is composed of an O polysaccharide (O antigen) an R core and the endotoxic inner Lipid A. Endotoxins evoke fever and can activate complement, kinin and clotting factors.

Applications:
Suitable for use in Immunofluorescence and ELISA. Other applications not tested.

Recommended Dilutions:
Immunofluorescence: 1:10-1:50
ELISA: 1:20-1:200
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. FITC conjugates are sensitive to light.
TypeIsotypeCloneGrade
MabIgG2a5E433Affinity Purified
SizeStorageShippingSourceHost
1ml-20CBlue IceMouse
Concentration:
~0.1mg/ml
Immunogen:
Salmonella
Purity:
Purified by Protein A chromatography.
Form
Supplied as a liquid in PBS, pH 7.2. No stabilizing proteins have been added. No preservative added. Labeled with FITC.
Specificity:
Recognizes common LPS core of all Salmonellae tested. Positive for all Salmonella O-serogroups tested: A, B, C1, C2, D, E1, E3, E4, F, G1 and G2. Negative for E. coli (mix), Klebsiella, Citrobacter, Pseudomonas, Yersinia, Shigella, Proteus and Legionella
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.