Technical Data
Selenoprotein M, Suberites domuncula (SelM, Sepn, 1500040L08Rik, A230103K18)
Selenium may be implicated to cancer prevention, immune system function, male fertility, cardiovascular disorder, control of the aging and neurodiseases process. Selenoproteins are thought to be responsible for the majority of these biomedical effects of selenium. Approximately 17 selenoproteins have been identified until now. Although the function of many selenoproteins are unknown, some play important roles in anti-oxidant mechanisms. It has been also implicated in the regulation of signaling pathways through catalysis of thiol/disulfide exchange. Selenoprotein M(SelM) is especially expressed in a mammalian brain and is localized to the perinuclear structures(Golgi/ER).

Suitable for use in ELISA, Western Blotting, Immunohistochemistry. Other applications not tested.

Recommended Dilution:
ELISA: 1:10,000-1:15,000
Western Blotting: 1:2000. Extracts from sodium selenite-treated primmorphs were separated by size and transferred to membranes.
Immunohistochemistry: 1:200
Optimal dilutions to be determined by the researcher.

Storage and Stability:
Lyophilized powder may be stored at 4°C for short-term only. Reconstitute to nominal volume by adding sterile 40-50% glycerol and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
20ul-20°CBlue IceRabbit
Recombinant selenoprotein M from Suberites domuncula
Supplied as a lyophilized powder in 50mM TBS, pH 7.2.
Selenoprotein M (Suberites domuncula)
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
Müller WEG, Borejko A, Brandt D, Osinga R, Ushijima H, Hamer B, Krasko A, Xupeng C, Müller I and Schröder HC (2005) Selenium affects biosilica formation in the demosponge Suberites domuncula: Effect on gene expression and spicule formation. FEBS Journal 272: 3838-3852