Technical Data
SAP102 (Synapse-associated Protein 102, bA291O7.3, Discs Large Drosophila Homolog 3, Discs Large Homolog 3, DLG3, KIAA1232, mKIAA1232, MRX, MRX90, Neuroendocrine dlg, NEDLG, NE-Dlg, XLMR)
Synapse-associated protein 102 belongs to the membrane-associated guanylate kinase protein
family and is a homolog of the Drosophila dis large tumor suppressor protein. SAP102 has extensive sequence homology to the PSD 95 family of proteins that facilitate ion channel clustering at synaptic terminal (1, 2). SAP102 consists of three 90 amino acids PDZ domains at its amino terminus, a Src homolgy 3 domain and a guanylate kinase domain (3). All three PDZ domains of SAP102 participate in binding to the NMDA reception, interacting specifically with
the carboxy-terminal domain of the N-methyl-D- aspartate receptor 2B (NR2B). The interaction may facilitate AMPA receptor withdrawal from the postsynaptic membrane by inhibiting the ERK/MAPK pathway (3, 4). SAP102 also interacts with synaptic ras-GTPase activating protein synGAP through its PDZ domains (3). In general, the SAP102 protein is more highly expressed in non-proliferating cells than in proliferating cells, indicating a role in the negative regulation of cell growth.

Suitable for use in Western Blot, Immunoprecipitation and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Western Blot: 1:1000
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
MabIgG110F763Affinity Purified
25ug-20CBlue IceRatMouse
Fusion protein aa 1-120 of rat SAP102 (accession number Q62936).
Purified by Protein G affinity chromatography.
Supplied as a liquid in PBS, pH 7.4, 0.09% sodium azide and 50% glycerol.
Recognizes rat sap102. Species Crossreactivity: human.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. 1. Muller B.M., et al. (1996) Neuron 17: 255-265. 2. 2. Lau L. F., et al. (1996) J. Biol. Chem. 271: 21622-21628. 3. 3. Cuthbert P.C., et al. (2007) J Neuroscience 27:2673-2682. 4. 4. Lau L.F., et al. (1996) J Biol Chem 274:5782-5790.