Technical Data
S0095-31
SAP97 (Synapse-associated Protein 97, Discs Large Homolog 1, DLG1, dJ1061C18.1.1, DKFZp761P0818, DLGH1, HDLG, Presynaptic Protein SAP97)
Description:
Synapse-associated protein (SAP) 97 is a 97 kD membrane-associated synapse protein with extensive sequence homology to the PSD 95 family of proteins that facilitate ion channel clustering at synaptic terminal (1). SAP97 consists of three 90 amino acid PDZ domains at its amino terminus, followed by a Src homology (SH) 3 domain, and a region with homology to guanylate kinases (GK). In addition to the CNS, SAP97 is also detected at the basal lateral membrane between a variety of epithelial cells. SAP97 is recruited to the submembranous cortical cytoskeleton at cell-cell contact sites as part of the E-cadherin complex. Co-expression of SAP97 and K+ channel results in the colocalization of these proteins in large round intracellular aggregates. SAP 97 has been shown to have functional homology to Drosophila tumor suppressor protein (DlgA), a PSD 95 related protein involved in maintaining integrity of epithelial and neuromuscular junctions.

Applications:
Suitable for use in Western Blot, Immunoprecipitation, Immunocytochemistry and Immunohistochemistry. Other applications have not been tested.

Recommended Dilutions:
Western Blot: 1:1000
Immunohistochemistry (Frozen): 1:1000
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgGPurified
SizeStorageShippingSourceHost
100ul-20CBlue IceRatRabbit
Concentration:
Not Determined
Immunogen:
Synthetic peptide corresponding to aa115-133, VLPSERISPQVPNEVLGPE of rat SAP97.
Purity:
Purified
Form
Supplied as a liquid in PBS, 0.05% sodium azide.
Specificity:
Recognizes rat SAP97 at ~140kD. Species Crossreactivity: mouse (liver).
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Muller, B.M., Kistner, U., Veh, R.W., Cases-Langhoff, C., Becker, B., Gundelfinger, E.D., Garner, C.C. (1995) J. Neurosci. 15: 2354-2366. 2. Reuver, S., Garner, C. (1998) J. Cell Sci. 111: 1071-1080. 3. Kim, E., and Sheng, M. (1996) Neuropharmacolog 35: 993-1000. 4. Thomas, U., Phannavong, B., Muller, B., Garner, C.C., Gundelfinger, E.D. (1997) Mech Dev. 62: 161-174. 5. D. Leonoudakis, et al.; J. Biol. Chem. 279, 19051 (2004)