Technical Data
S0096-04A
SAPK, JNK , phosphorylated (Thr183 Tyr185)(Stress-Activated Protein Kinase, Jun-terminal Kinase)
Description:
The stress-activated protein kinase/Jun-amino-terminal kinase SAPK/JNK is potently and preferentially activated by a variety of environmental stresses including UV and gamma radiation, ceramides, inflammatory cytokines and in some instances, by growth factors and GPCR agonists (1-6). As with the other MAPKs, the core signaling unit is composed of a MAPKKK, typically MEKK1-MEKK4, or by one of the mixed lineage kinases (MLKs), which phosphorylate and activate MKK4/7. Upon activation, MKKs phosphorylate and activate the SAPK/JNK kinase (2). Stress signals are delivered to this cascade by small GTPases of the Rho family (Rac, Rho, cdc42) (3). Both Rac1 and cdc42 mediate the stimulation of MEKKs and MLKs (3). Alternatively, MKK4/7 can be activated in a GTPase independent mechanism via stimulation of a germinal center kinase (GCK) family member (4). There are three SAPK/JNK genes each of which undergoes alternative splicing resulting in numerous isoforms (3). SAPK/JNK, when active as a dimer, can translocate to the nucleus and regulate transcription through its effects on c-Jun, ATF-2 and other transcription factors (3,5).

Applications:
Suitable for use in ELISA, Western Blot, Immunoprecipitation and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Western Blot: 1:1000, incubate membrane with diluted antibody in 5% BSA, 1X TBS, 0.1% Tween-20 at 4C with gentle shaking, overnight.
Immunoprecipitation: 1:200
Immunohistochemistry (Paraffin): 1:100
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage, store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
200ul4C (-20C Glycerol)Blue IceHumanRabbit
Concentration:
~1mg/ml
Immunogen:
Synthetic phosphopeptide corresponding to residues surrounding Thr183/Tyr185 of human SAPK/JNK (KLH).
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as liquid in 10mM HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol. No preservative added.
Specificity:
Recognizes endogenous levels of human p46 and p54 SAPK/JNK dually phosphorylated at threonine 183 and tyrosine 185 between 46-54kD. Does not recognize unphosphorylated SAPK/JNK. Species Crossreactivity: mouse, monkey, hamster, bovine, rat, Drosophila. Species sequence homology: Xenopus
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Davis, R.J. (1999) Biochem. Soc. Symp. 64, 112. 2. Ichijo, H. (1999) Oncogene 18, 60876093. 3. Kyriakis, J.M. and Avaruck, J. (2001) Physiol. Rev. 81, 807869. 4. Kyriakis, J.M. (1999) J. Biol. Chem. 274, 52595262. 5. Leppa, S. and Bohmann, D. (1999) Oncogene 18, 61586162. 6. Whitmarsh, A.J. and Davis, R.J. (1998) Trends Biochem. Sci. 23, 481485. 7. Kujime, K. et al. (2000) J. Immunol. 164, 32223228. 8. Rochat-Steiner, V. et al. (2000) J. Exp. Med. 192, 11651174. 9. Shukla, A. et al. (2001) Cancer Res. 61, 17911795. 10. Weiss, L. et al. (2000) J. Exp. Med. 191, 139146.