Technical Data
S0096-04C
SAPK, JNK, phosphorylated (Thr183 Tyr185) (Stress-Activated Protein Kinase, Jun-terminal Kinase)
Description:
The stress-activated protein kinase/Junterminal kinase SAPK/JNK is potently and preferentially activated by a variety of environmental stresses, including UV and gamma radiation, ceramides, inflammatory cytokines and, in some instances, by growth factors and GPCR agonists. As with the other MAPKs, the core signaling unit is composed of an MAPKKK, typically MEKK1-4, or a member of the mixed lineage kinases (MLKs) that would phosphorylate and activate MKK4-7, the SAPK/JNK kinase. Stress signals are delivered to this cascade by members of small GTPases of the Rho family (Rac, Rho, cdc42). Both Rac1 and cdc42 mediate the stimulation of MEKKs and MLKs. Alternatively, MKK4-7 can be activated in a pathway independent of small GTPases via stimulation of a member of the germinal center kinase (GCK) family. There are three SAPK/JNK genes with further diversification from alternative splicing. SAPK/JNK, when active as a dimer, can translocate to the nucleus where it regulates transcription through its effects on c-Jun, ATF-2 and other transcription factors.

Application:
Suitable for use in ELISA, Western Blot, Immunoprecipitation, and Flow Cytometry. Other applications not tested.

Recommended Dilution:
Western Blot: 1:2000. Incubate membrane with diluted S0096-04C in 1X TBS, 5% nonfat dry milk, 0.1% Tween-20 at 4C with gentle shaking, overnight.
Immunoprecipitation: 1:250
Flow Cytometry: 1:200
Optimal dilutions to be determined by the researcher.

Source: Ascites

Storage and Stability:
For long-term storage, aliquot and store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
MabIgG13A33Affinity Purified
SizeStorageShippingSourceHost
200ul4C (-20C Glycerol)Blue IceHumanMouse
Concentration:
Not determined
Immunogen:
Synthetic phosphopeptide corresponding to residues around Thr183/Tyr185 of human SAPK/JNK (KLH coupled).
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as liquid in 10mM HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol. No preservative added.
Specificity:
Detects endogenous levels of human p46 and p54 SAPK/JNK dually phosphorylated at threonine 183 and tyrosine 185. Does not recognize endogenous levels of phosphorylated p44/42 MAPK or p38 MAP kinase. Species Crossreactivity: Human, mouse and rat.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Davis, R.J., Biochem. Soc. Symp. 64: 1-12 (1999). 2. Ichijo, H., Oncogene 18: 6087-6093 (1999). 3. Kyriakis, J.M., Avaruck, J., Physiol. Rev. 81: 807-869 (2001). 4. Kyriakis, J.M., J. Biol. Chem. 274: 5259-5262 (1999). 5. Leppa, S., Bohmann, D., Oncogene 18: 6158-6162 (1999). 6. Whitmarsh, A.J., Davis, R.J., Trends Biochem. Sci. 23: 481-485 (1998). 7. Akagi, T., et al., PNAS USA 97: 7290-7295 (2000). 8. Saporito, M.S., et al., J. Neurochem. 75: 1200-1208 (2000). 9. Somasundaram, S., et al., Cancer Res. 62: 3868-3875 (2002). 10. Song, J.S., et al., J. Immunol. 163: 802-810 (1999). 11. Xing, J., et al., Mol. Cell. Biol. 18: 1946-1955 (1998). 12. Zhang, S., Kaplan, M.H., J. Immunol. 165: 1374-1380 (2000).