Technical Data
S0096-04E
SAPK, JNK (Stress-Activated Protein Kinase, Jun-terminal Kinase)
Description:
The stress-activated protein kinase/Jun terminal kinase SAPK/JNK is potently and preferentially activated by a variety of environmental stresses, including UV and gamma radiation, ceramides, inflammatory cytokines and, in some instances, by growth factors and GPCR agonists. As with the other MAPKs, the core signaling unit is composed of an MAPKKK, typically MEKK14, or a member of the mixed lineage kinases (MLKs) that would phosphorylate and activate MKK47, the SAPK/JNK kinase. Stress signals are delivered to this cascade by members of small GTPases of the Rho family (Rac, Rho, cdc42). Both Rac1 and cdc42 mediate the stimulation of MEKKs and MLKs. Alternatively, MKK47 can be activated in a pathway independent of small GTPases via stimulation of a member of the germinal center kinase (GCK) family. There are three SAPK/JNK genes with further diversification from alternative splicing. SAPK/JNK, when active as a dimer, can translocate to the nucleus where it regulates transcription through its effects on c-Jun, ATF-2 and other transcription factors.

Applications:
Suitable for use in Western Blot, Immunocytochemistry and Flow Cytometry. Other applications have not been tested.

Recommended Dilution:
Western Blot: 1:1000, incubate membrane with diluted antibody in 5% BSA, 1X TBS, 0.1% Tween-20 at 4C with gentle shaking, overnight.
Immunocytochemistry (ABC): 1:200
Flow Cytometry: 1:25
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage, store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
200ul4C (-20C Glycerol)Blue IceHumanRabbit
Concentration:
Not determined
Immunogen:
Fusion protein of human JNK2 and GST.
Purity:
Purified by Protein A affinity chromatography.
Form
Supplied as liquid in 10mM HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol. No preservative added.
Specificity:
Detects endogenous levels of total SAPK/JNK protein. Species Crossreactivity: Human, mouse, rat and zebrafish.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Davis, R.J. (1999) Biochem. Soc. Symp. 64, 112. 2. Ichijo, H. (1999) Oncogene 18, 60876093.
3. Kyriakis, J.M. and Avaruck, J. (2001) Physiol. Rev. 81, 807869. 4. Kyriakis, J.M. (1999) J. Biol. Chem. 274, 52595262. 5. Leppa, S. and Bohmann, D. (1999) Oncogene 18, 61586162. 6. Whitmarsh, A.J. and Davis, R.J. (1998) Trends Biochem. Sci. 23, 481485. 7. Banerjee, S. et al. (2002) J. Virol. 76, 59375948. 8. Brouard, S. et al. (2000) J. Exp. Med. 192, 10151026. 9. Chen, F. et al. (2001) J. Biol. Chem. 276, 1141411419. 10. Dai, Y. et al. (2001) Cancer Res. 61, 51065115. 11. Hebert, S.S. et al. (2000) J. Biol. Chem. 275, 3248232490. 12. Lee, E.G. et al. (2000) Science 289, 23502354. 13. Park, Y.C. et al. (2000) Cell 101, 777787.