Technical Data
S0096-23B
SF3b155/SAP155 (Pre-mRNA-splicing factor SF3b 155kD subunit, Prp10, PRP10, PRPF10, SAP155, SAP 155, SF3b155, Spliceosome-associated protein 155, Splicing factor 3B subunit 1)
Description:
SAP155 is a subunit of the splicing factor SF3B required for 'A' complex assembly formed by the stable binding of U2 snRNP to the branchpoint sequence (BPS) in pre-mRNA. Sequence independent binding of the SF3A/SF3B complex upstream of the branch site is essential, it may anchor U2 snRNP to the pre-mRNA. It may also be involved in the assembly of the 'E' complex. SAP155 also belongs to the minor U12-dependent spliceosome, which is involved in the splicing of rare class of nuclear pre-mRNA intron.

Applications:
Suitable for use in Immunoprecipitation and Western Blot. Other applications have not been tested.

Recommended Dilution:
Immunoprecipitation: 2-5ug/mg
Western Blot: 1:2000-1:10,000
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabAffinity Purified
SizeStorageShippingSourceHost
100ul-20CBlue IceHumanRabbit
Concentration:
~0.2mg/ml
Immunogen:
The epitope recognized by this antibody maps to a region between residue 100 and 150 of human splicing factor 3b, subunit 1, 155kD using the numbering given in entry NP_036565.2 (GeneID 23451).
Purity:
Purified by Immunoaffinity chromatography.
Form
Supplied as a liquid in TBS, pH 7.2, 0.1% BSA, 0.09% sodium azide.
Specificity:
Recognizes human SF3b155.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.