Technical Data
SAPK2, p38 Inhibitor (SB 203580) (Stress-activated Protein Kinase 2)
Molecular Biology Storage: -20°CShipping: Blue Ice
SB 203580 is a highly specific inhibitor of SAPK2a/p38 (IC50=600nM). It suppresses the activation of MAPKAP Kinase-2 and MAPKAP Kinase-3. SB 203580 does not significantly inhibit SAPK/JNK or Erk/MAP kinases at 100mM. It inhibits IL-1 and TNF-a production from LPS-stimulated human monocytes and the human monocyte cell line THP-1. SB 203580 has been shown to inhibit activation of the HIV-1 long terminal repeat by IL-1 and TNF-a. It has also been shown to be an effective inhibitor of inflammatory cytokine production in vivo in mice and rats.

Applications: Suitable for use in Protein Kinase Inhibition Assay. Other applications not tested.

Recommended Dilutions: Protein Kinase Inhibition Assay: A 20 minute pre-incubation of immunoprecipitated SAPK2a/p38 MAPK with 1-10uM of SAPK3/p38 Inhibitor (SB203580) inhibits the kinase activity 80-95%. Optimal concentration of SB 203580 needed may depend on the amount of enzyme used.

Storage and Stability:
Lyophilized powder may be stored at 4°C for short-term only. For Long term storage, store at -20°C. Lyophilized powder is stable for 36 months at -20°C. Reconstitute to nominal volume by adding sterile DMSO, aliquot and store at -20°C. Reconstituted product is stable for 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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Source: Human, synthetic protein.

Purity: 98% by HPLC

Solubility: Soluble in DMSO at 50mg/ml.

Form: Supplied as a pale yellow lyophilized powder.
1. Badger, A.M., et al., J. Pharmcol Exp. Therap. 279: 1453, 1996. 2. Hazzalin, C.A., et al., Curr. Biol. 6: 1028, 1996. 3. Kramer, R.M., et al., J. Biol. Chem. 271: 27723, 1996. 4. Saklatvala, J., et al., J. Biol. Chem. 271: 6586, 1996. 5. Cuenda, A., et al., FEBS Lett. 364: 229, 1995. 6. Gallagher, T.F., et al., Bioorg. Med. Chem. Lett. 5: 1171, 1995. 7. Lee, J.C., et al., Nature. 372: 739, 1994.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.