Technical Data
S0096-27M
SAPK2a, p38, NT (Stress-activated Protein Kinase 2a)
Description:
Stress-activated Protein Kinases (SAPKs) are strongly activated in response to adverse stimuli such as heat and osmotic shock, UV light and other DNA damaging reagents, and inhibitors of protein synthesis. They are also activated strongly in response to agonists that are released or produced under conditions of stress, such as proinflammatory cytokines. Stress-activated Protein Kinase 2 (SAPK2/p38) phosphorylates conserved Ser-Pro or Thr-Pro peptide motifs.

Applications:
Suitable for use in ELISA and Western Blot. Other applications have not been tested.

Recommended Dilution:
ELISA: 0.05ug/ml
Western Blot: 0.1ug/ml for HRP/ECL detection.
Optimal dilutions to be determined by researcher.

Positive Control (included):
A431 cell lysate, untreated (A0001-50B).

Storage and Stability:

May be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
MabIgG1,k6D752Affinity Purified
SizeStorageShippingSourceHost
50ug4°C (-20°C Glycerol)Blue IceHumanMouse
Concentration:
~0.1mg/ml
Immunogen:
Synthetic peptide corresponding to N-terminal SAPK2a (p38) (KLH).
Purity:
Purified by thiophilic adsorption and size exclusion chromatography.
Form
Supplied as a liquid in PBS, pH 7.4, 0.1% sodium azide, PEG and sucrose.
Specificity:
Recognizes human SAPK2a(p38). Does not cross-react with the splice variant Mxi2. Species Crossreactivity: mouse.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.