		Technical Data
S0096-28	SAPK2a, p38 alpha, active (Stress-activated Protein Kinase 2a) Recombinant	10ug

Molecular Biology

Storage: -20°CShipping: Blue Ice

GST fusion protein containing full length human SAPK2a/p38a (64kDa).

Specificity: Essentially identical in mammals (p38 MAPK) and yeast (HOG-1). Inhibitors include protein phosphatase 2A, PTP-1B, CL-100 and pyridinyl imidazols.

Specific Activity: 50units/mg using MBP as a substrate. One unit of SAPK2a/p38a = 1 nmol phosphate incorporated into MBP per minute.

SAPK2a Kinase Assay: SAPK2a/p38a phosphorylated MBP.

Storage and Stability: May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Source: Human recombinant expressed in E. coli

Purity: 98% by SDS and Coomassie Blue staining

Form: Supplied as a liquid in 50mM Tris-HCl, pH 7.5, 150mM sodium chloride, 0.1mM EGTA, 0.03% Brij-35, 1mM benzamidine, 0.2mM PMSF, 0.1% 2-mercaptoethanol, 50% glycerol.

Intended for research use only, not for use in human, therapeutic or diagnostic applications.

1. Doza, Y.N., et al., FEBS Lett. 364: 223–228, 1995. 2. Ben-Levy, et al., EMBO. J. 13: 3302–3311, 1994. 3. Cuenda, A., et al., FEBS Lett. 364: 229–231, 199

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.