Technical Data
S0096-54
SAPK4, p38 delta (Stress-activated Protein Kinase 4, Mitogen-activated Protein Kinase 13, MAP Kinase 13, MAPK 13, MAPK13, PRKM13, Mitogen-activated Protein Kinase p38 delta, MAP Kinase p38 delta, p38delta)
Description:
Stress-activated Protein Kinases (SAPKs) are strongly activated in response to adverse stimuli such as heat and osmotic shock, UV light and other DNA-damaging reagents, and inhibitors of protein synthesis. They are also activated strongly in response to agonists that are released or produced under conditions of stress, such as proinflammatory cytokines. Stress-activated Protein Kinase 2 (SAPK2/p38) phosphorylates conserved Ser-Pro or Thr-Pro peptide motifs.

Applications:
Suitable for use in ELISA and Western Blot. Other applications not tested.

Recommended Dilution:
ELISA: 0.05ug/ml
Western Blot: 1ug/ml (Alkphos detection), 0.1ug/ml (HRP/ECL detection).
Optimal dilutions to be determined by the researcher.

Positive Control (included):
A0001-50B A431 cell lysate, untreated
Add 75ul 2x SDS-PAGE sample buffer, mix and incubate at 90C for 5 min.
Western Blot: 5ul/lane (mini gel) for HRP/ECL detection; 20ul/lane (mini gel) for HRP/DAB detection.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgG16D753
SizeStorageShippingSourceHost
50ug-20CBlue IceHumanMouse
Concentration:
~0.1mg/ml
Immunogen:
Synthetic peptide corresponding to a portion of N-terminal SAPK4 (KLH).
Purity:
Purified by thiophilic adsorption and size-exclusion chromatography.
Form
Supplied as a liquid in PBS, PEG, sucrose, 0.1% sodium azide.
Specificity:
Recognizes N-terminal human, mouse and rat p38 . Species Crossreactivity: Human, mouse, rat
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.