Technical Data
S0096-54
SAPK4, p38 delta (Stress-activated Protein Kinase 4, Mitogen-activated Protein Kinase 13, MAP Kinase 13, MAPK 13, MAPK13, PRKM13, Mitogen-activated Protein Kinase p38 delta, MAP Kinase p38 delta, p38delta)
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Technical Data |
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S0096-54 |
SAPK4, p38 delta (Stress-activated Protein Kinase 4, Mitogen-activated Protein Kinase 13, MAP Kinase 13, MAPK 13, MAPK13, PRKM13, Mitogen-activated Protein Kinase p38 delta, MAP Kinase p38 delta, p38delta) |
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Description: Stress-activated Protein Kinases (SAPKs) are strongly activated in response to adverse stimuli such as heat and osmotic shock, UV light and other DNA-damaging reagents, and inhibitors of protein synthesis. They are also activated strongly in response to agonists that are released or produced under conditions of stress, such as proinflammatory cytokines. Stress-activated Protein Kinase 2 (SAPK2/p38) phosphorylates conserved Ser-Pro or Thr-Pro peptide motifs. Applications: Suitable for use in ELISA and Western Blot. Other applications not tested. Recommended Dilution: ELISA: 0.05ug/ml Western Blot: 1ug/ml (Alkphos detection), 0.1ug/ml (HRP/ECL detection). Optimal dilutions to be determined by the researcher. Positive Control (included): A0001-50B A431 cell lysate, untreated Add 75ul 2x SDS-PAGE sample buffer, mix and incubate at 90°C for 5 min. Western Blot: 5ul/lane (mini gel) for HRP/ECL detection; 20ul/lane (mini gel) for HRP/DAB detection. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. |
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