		Technical Data
S0097-70D	SARM, CT (Sterile alpha and TIR Motif-containing Protein 1, SARM1, FLJ36296, KIAA0524, Sterile alpha and Armadillo Repeat Protein, Sterile alpha Motif Domain-containing Protein 2, SAM Domain-containing Protein 2, SAMD2, Tir-1 Homolog)

Description:
Toll-like receptors (TLRs) are signaling molecules that recognize different microbial products during infection and serve as an important link between the innate and adaptive immune responses. SARM (SAM and ARM-containing protein), along with other molecules such as TIRP, TRIF, TIRAP, and MyD88, is thought to serve as an adaptor protein for the TLRs that allows for the activation of downstream kinases and NF-?B, andultimately the expression of proteins involved in host defense. While SARM has not been conclusively shown to associate directly with TLRs, the presence of a Toll-interluekin-1 (TIR) domain in SARM is consistent with a role as a signaling molecule.

Applications:
Suitable for use in Immunofluorescence, Western Blot and Immunocytochemistry. Other applications not tested.

Recommended Dilution:
Immunofluorescence: 2ug/ml
Western Blot: 0.5-2ug/ml
Immunocytochemistry: 2ug/ml
Optimal dilutions to be determined by the researcher.

Positive Control:
Daudi cell lysates.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Type	Isotype	Clone	Grade
Pab	IgG		Purified

Size		Storage	Shipping	Source	Host
50ug		-20°C	Blue Ice	Human	Rabbit

Concentration:

As reported

Immunogen:

Synthetic peptide corresponding to human SARM (CT).

Purity:

Purified

Form

Supplied as a liquid in PBS, 0.02% sodium azide.

Specificity:

Recognizes human SARM (CT). Species Crossreactivity: mouse.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

1. Vogel SN, Fitzgerald KA, and Fenton MJ. TLRs: differential adapter utilization by toll-like receptors mediates TLR-specific patterns of gene expression. Mol. Interv. 2003; 3:466-77. 2. Takeda K, Kaisho T, and Akira S. Toll-like receptors. Annu. Rev. Immunol. 2003; 21:335-76. 3. Janeway CA Jr and Medzhitov R. Innate immune recognition. Annu. Rev. Immunol. 2002; 20:197-216. 4. O’Neill LAJ, Fitzgerald FA, and Bowie AG. The Toll-IL-1 receptor adaptor family grows to five members. Trends in Imm. 2003; 24:286-9. 5. McGettrick AF and O’Neill LAJ. The expanding family of MyD88-like adaptors in Toll-like receptor signal transduction. Mol Imm. 2004; 41:577-82.