Description:
A novel coronavirus has recently been identified as the causative agent of SARS (Severe Acute Respiratory Syndrome) (1-2). Coronaviruses are a major cause of upper respiratory diseases in humans (3). The genomes of these viruses are positive-stranded RNA approximately 27-31kb in length. The SARS E protein contains a short palindromic transmembrane helical hairpin that seems to deform lipid bilayers (4), which may explain its role in viral budding and virion envelope morphogenesis (5).
Applications:
Can be used for the detection of SARS E protein in ELISA. It will detect 10 ng of free peptide at 1 mg/ml.
Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Store at -20°C or colder. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
|
| Type | Isotype | Clone | Grade |
|---|
| Pab | IgG | | Affinity Purified |
|
| Size | | Storage | Shipping | Source | Host |
|---|
| 100ug | | -20°C | Blue Ice | | Rabbit |
|
| Concentration: |
| ~0.5mg/ml |
| Immunogen: |
| Raised against a synthetic peptide corresponding to amino acids at the carboxy-terminus of the SARS E protein (Genbank accession no. P59637) |
| Purity: |
| Purified by immunoaffinity chromatography. |
| Form |
| Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide. |
| Specificity: |
| Recognizes SARS E protein. |
|
| Intended for research use only. Not for use in human, therapeutic, or diagnostic applications. |
1. Marra MA, Jones SJ, Astell CR, et al. The Genome sequence of the SARS-associated corona virus. Science 2003;300:1399-404. 2. Rota PA, Oberste MS, Monroe SS, et al. Characterization of a novel coronavirus associated with severe acute respiratory syndrome. Science 2003;300:1394-9. 3. Navas-Nartin SR and Weiss S. Coronavirus replication and pathogenesis: Implications for the recent outbreak of severe acute respiratory syndrome (SARS), and the challenge for vaccine development. J Neurovirol. 2004;10:75-85. 4. Arbely E, Khattari Z, Brotons G, et al. A highly unusual palindromic transmembrane helical hairpin formed by SARS coronavirus E protein. J Mol. Biol. 2004;3414:769-79. 5. Maeda J, Maeda A, and Makino S. Release of coronavirus E protein in membrane vesicles from virusinfected cells and E protein-expressing cells. Virology 1999;263:265-72.
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