Technical Data
SARS E Protein (Severe Acute Respiratory Syndrome)
A novel coronavirus has recently been identified as the causative agent of SARS (Severe Acute Respiratory Syndrome) (1-2). Coronaviruses are a major cause of upper respiratory diseases in humans (3). The genomes of these viruses are positive-stranded RNA approximately 27-31kb in length. The SARS E protein contains a short palindromic transmembrane helical hairpin that seems to deform lipid bilayers (4), which may explain its role in viral budding and virion envelope morphogenesis (5).

Can be used for the detection of SARS E protein in ELISA. It will detect 10 ng of free peptide at 1 mg/ml.

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Store at -20C or colder. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
PabIgGAffinity Purified
100ug-20CBlue IceRabbit
Raised against a synthetic peptide corresponding to amino acids at the carboxy-terminus of the SARS E protein (Genbank accession no. P59637)
Purified by immunoaffinity chromatography.
Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide.
Recognizes SARS E protein.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Marra MA, Jones SJ, Astell CR, et al. The Genome sequence of the SARS-associated corona virus. Science 2003;300:1399-404. 2. Rota PA, Oberste MS, Monroe SS, et al. Characterization of a novel coronavirus associated with severe acute respiratory syndrome. Science 2003;300:1394-9. 3. Navas-Nartin SR and Weiss S. Coronavirus replication and pathogenesis: Implications for the recent outbreak of severe acute respiratory syndrome (SARS), and the challenge for vaccine development. J Neurovirol. 2004;10:75-85. 4. Arbely E, Khattari Z, Brotons G, et al. A highly unusual palindromic transmembrane helical hairpin formed by SARS coronavirus E protein. J Mol. Biol. 2004;3414:769-79. 5. Maeda J, Maeda A, and Makino S. Release of coronavirus E protein in membrane vesicles from virusinfected cells and E protein-expressing cells. Virology 1999;263:265-72.