Technical Data
SARS, Nucleocapsid Protein (Severe Acute Respiratory Syndrome)
It has recently been shown that SARS is caused by a human coronavirus. Human coronaviruses are the major cause of upper respiratory tract illness in humans, such as the common cold. Coronaviruses are positive-stranded RNA viruses, featuring the largest viral RNA genomes known to date (27-31 kb). The first step in coronavirus infection is binding of the viral spike protein, a 139-kD protein, to certain receptors on host cells. The spike protein is the main surface antigen of the coronavirus. The most prominent protein in the culture supernatants infected with SARS virus is a 46kD nucleocapsid protein. This suggests that the nucleocapsid protein is a major immunogen that may be useful for early diagnostics.

Suitable for use in Western Blot.
Recommended Dilution:
Western Blot: 1:2000
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
100ug-20CBlue IceRabbit
As reported
Synthetic peptides corresponding to amino acids 20-34 of putative SARS nucleocapsid (Genbank accession no. NP_828858).
Supplied as a liquid in PBS, 0.2% gelatin, 0.05% sodium azide.
Recognizes SARS, Nucleocapsid Protein (Severe Acute Respiratory Syndrome)
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
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4. Snijder,E.J., Bredenbeek,P.J., Dobbe,J.C., Thiel,V., Ziebuhr,J., et al. J. Mol. Biol. 331 (5), 991-1004 (2003).