Technical Data
S0098-10G
SARS, Nucleocapsid Protein (Severe Acute Respiratory Syndrome, N Protein, N Structural Protein, NC, SARS Coronavirus N Protein, SARS CoV N Protein, SARS-CoV N Protein, SARS Coronavirus Nucleocapsid Protein, SARS CoV Nucleocapsid Protein, SARS-CoV Nucleoca
Description:
It has recently been shown that SARS is caused by a human coronavirus. Human coronaviruses are the major cause of upper respiratory tract illness in humans, such as the common cold. Coronaviruses are positive-stranded RNA viruses, featuring the largest viral RNA genomes known toDte (27-31kb). The first step in coronavirus infection is binding of the viral spike protein, a 139kD protein, to certain receptors on host cells. The spike protein is the main surface antigen of the coronavirus. The most prominent protein in the culture supernatants infected with SARS virus is a 46kD nucleocapsid protein. This suggests that the nucleocapsid protein is a major immunogen that may be useful for early diagnostics.

Applications:
Suitable for use in Western Blot. Other applications not tested.

Recommended Dilution:
Western Blot: 1:500-1:1000
Optimal dilutions to be determined by the researcher.

Positive Control:
Transfected cell line

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgM11E343Affinity Purified
SizeStorageShippingSourceHost
200ul-20CBlue IceMouse
Concentration:
Not determined
Immunogen:
Synthetic peptide corresponding to aa354-370 of SARS nucleocapsid protein (KLH).
Purity:
Purified by Protein G affinity chromatography.
Form
Supplied as a liquid in PBS, 0.2% gelatin, 0.05% sodium azide.
Specificity:
Recognizes SARS, Nucleocapsid Protein.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Snijder,E.J., Bredenbeek,P.J., Dobbe,J.C., Thiel,V., Ziebuhr,J., et al. J. Mol. Biol. 331 (5), 991-1004 (2003). 2. Marra MA, Jones SJ, Astell CR, Holt RA, Brooks-Wilson A, Butterfield YS, Khattra J, Asano JK, Barber SA, Chan SY, et al. Science. 300(5624):1399-1404 (2003). 3. Rota PA, Oberste MS, Monroe SS, Nix WA, Campagnoli R, et al. Science. 300(5624):1394-1399 (2003). 4. Krokhin O, Li Y, Andonov A, Feldmann H, Flick R, Jones S, Stroeher U. Mol Cell Proteomics. May;2(5):346-356 (2003).