Technical Data
S0098-11
SARS, Nucleocapsid Protein (Severe Acute Respiratory Syndrome)
Description:
It has recently been shown that SARS is caused by a human coronavirus. Human coronaviruses are the major cause of upper respiratory tract illness in humans, such as the common cold. Coronaviruses are positive-stranded RNA viruses, featuring the largest viral RNA genomes known to date (27-31 kb). The first step in coronavirus infection is binding of the viral spike protein, a 139-kD protein, to certain receptors on host cells. The spike protein is the main surface antigen of the coronavirus. The most prominent protein in the culture supernatants infected with SARS virus is a 46kD nucleocapsid protein. This suggests that the nucleocapsid protein is a major immunogen that may be useful for early diagnostics.

Applications:
Suitable for the detection of SARS Nucleocapsid protein in western blot analysis and ELISA. The antibody was tested on a cell line transfected with full-length SARS Nucleocapsid cDNA with a predicted molecular weight 46kD. Western blot analysis of SARS Nucleocapsid in (A) transfected mouse melanoma cell lysate and (B) untransfected cell lysate using antibody at a 1:2000 dilution.

Recommended Dilution:
Western blot: 1:100-1:2000
ELISA: 1:100-1:2000

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

TypeIsotypeCloneGrade
PabIgGPurified
SizeStorageShippingSourceHost
200ul-20CBlue IceRabbit
Concentration:
Not determined
Immunogen:
Synthetic peptides corresponding to amino acids 84-99 of putative SARS nucleocapsid (Genbank accession no. NP_828858).
Purity:
Purified
Form
Supplied as a liquid in PBS, 0.2% gelatin, 0.05% sodium azide.
Specificity:
Recognizes SARS, Nucleocapsid Protein (Severe Acute Respiratory Syndrome)
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Marra MA, Jones SJ, Astell CR, Holt RA, Brooks-Wilson A, Butterfield YS, Khattra J, Asano JK, Barber SA, Chan SY, et al. Science. 300(5624):1399-1404 (2003).
2. Rota PA, Oberste MS, Monroe SS, Nix WA, Campagnoli R, et al. Science. 300(5624):1394-1399 (2003).
3. Krokhin O, Li Y, Andonov A, Feldmann H, Flick R, Jones S, Stroeher U. Mol Cell Proteomics. May;2(5):346-356 (2003).
4. Snijder,E.J., Bredenbeek,P.J., Dobbe,J.C., Thiel,V., Ziebuhr,J., et al. J. Mol. Biol. 331 (5), 991-1004 (2003).