Technical Data
S0098-11
SARS, Nucleocapsid Protein (Severe Acute Respiratory Syndrome)
|
Technical Data |
|
S0098-11 |
SARS, Nucleocapsid Protein (Severe Acute Respiratory Syndrome) |
|
|
Description: It has recently been shown that SARS is caused by a human coronavirus. Human coronaviruses are the major cause of upper respiratory tract illness in humans, such as the common cold. Coronaviruses are positive-stranded RNA viruses, featuring the largest viral RNA genomes known to date (27-31 kb). The first step in coronavirus infection is binding of the viral spike protein, a 139-kD protein, to certain receptors on host cells. The spike protein is the main surface antigen of the coronavirus. The most prominent protein in the culture supernatants infected with SARS virus is a 46kD nucleocapsid protein. This suggests that the nucleocapsid protein is a major immunogen that may be useful for early diagnostics. Applications: Suitable for the detection of SARS Nucleocapsid protein in western blot analysis and ELISA. The antibody was tested on a cell line transfected with full-length SARS Nucleocapsid cDNA with a predicted molecular weight 46kD. Western blot analysis of SARS Nucleocapsid in (A) transfected mouse melanoma cell lysate and (B) untransfected cell lysate using antibody at a 1:2000 dilution. Recommended Dilution: Western blot: 1:100-1:2000 ELISA: 1:100-1:2000 Storage and Stability: May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. |
|
|||||||||||
|
||||||||||||
|
||||||||||||
|
||||||||||||