Technical Data
SARS Nucleoprotein (Severe Acute Respiratory Syndrome)
The Coronaviridae virus family is characterized by a nucleocapsid that is composed of the genome RNA molecule in combination with the nucleoprotein (N protein) within a virion. The N protein (nucleoprotein) is one of the major structural proteins in a viral particle, playing a critical role in the transcription regulation of the genomic RNA and other viral proteins. It might also be involved in the virulence and virus-specific post-translational modifications. The N protein is composed of 422 amino acids (a.a.) with an estimated molecular weight of 46.03 kD. The most striking physiochemical feature of the N protein of SARS-CoV is that it is a typical basic protein with a high predicted pI and high hydrophilicity, which is consistent with its function of binding to the ribophosphate backbone of the RNA molecule. The N protein contains multiple candidate phosphorylation sites, suggesting that it may be related to important functions, such as
RNA-binding and localization to the nucleolus of host cells. Subsequent study shows that there is an SR-rich region in the N protein and this region might be involved in the protein-protein interaction. The abundant antigenic sites predicted in the N protein, as well as experimental evidence with synthesized polypeptides, indicate that the N protein is one of the major antigens of the SARS-CoV. Compared with other viral structural proteins, the low variation rate of the N protein with regards to its size suggests its importance to the survival of the virus.

Suitable for use in Immunofluorescence, Western Blot and ELISA. Other applications have not been tested.

Recommended Dilution:
Immunofluorescence: 1-5ug/ml
Western Blot: 1-5ug/ml
ELISA: 0.1-2ug/ml
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

MabIgG2a6A181Affinity Purified
100ug4C (-20C Glycerol)Blue IceHumanMouse
Recombinant SARS (severe acute respiratory syndrome) nucleoprotein.
Purified by Protein A chromatography.
Supplied as a liquid in PBS, pH 7.4, containing 0.09% sodium azide
Recogizes the human SARS-CoV nucleoprotein. On Western blots, it identifies the target band at ~50kD. Does not crossreact with the nucleoprotein of 229E-CoV or DC43-CoV. Reactivity has also been confirmed with SARS infected MDCK cells by immunofluorscence.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.