		Technical Data
S0098-19B	SARS Nucleoprotein (Severe Acute Respiratory Syndrome)

Description:
The Coronaviridae virus family is characterized by a nucleocapsid that is composed of the genome RNA molecule in combination with the nucleoprotein (N protein) within a virion. The N protein (nucleoprotein) is one of the major structural proteins in a viral particle, playing a critical role in the transcription regulation of the genomic RNA and other viral proteins. It might also be involved in the virulence and virus-specific post-translational modifications. The N protein is composed of 422 aas (a.a.) with an estimated molecular weight of 46.03 kD. The most striking physiochemical feature of the N protein of SARS-CoV is that it is a typical basic protein with a high predicted pI and high hydrophilicity, which is consistent with its function of binding to the ribophosphate backbone of the RNA molecule. The N protein contains multiple candidate phosphorylation sites, suggesting that it may be related to important functions, such as RNA-binding and localization to the nucleolus of host cells. Subsequent study shows that there is an SR-rich region in the N protein and this region might be involved in the protein-protein interaction. The abundant antigenic sites predicted in the N protein, as well as experimental evidence with synthesized polypeptides, indicate that the N protein is one of the major antigens of the SARS-CoV. Compared with other viral structural proteins, the low variation rate of the N protein with regards to its size suggests its importance to the survival of the virus.

Applications:
Suitable for use in Immunofluorescence, ELISA, and Western Blot. Other applications not tested.

Recommended Dilution:
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Type	Isotype	Clone	Grade
Mab	IgG2b	9L646	Affinity Purified

Size		Storage	Shipping	Source	Host
100ug		-20°C	Blue Ice		Mouse

Concentration:

As reported

Immunogen:

Recombinant SARS nucleoprotein.

Purity:

Purified by Protein A affinity chromatography.

Form

Supplied as a liquid in 0.01M PBS, pH 7.2, 0.1% sodium azide.

Specificity:

Recognizes the nucleoprotein of the SARS virus. Does not react with human coronaviruses 229E and OC43 as well as FIP-1, FIP 2, canine coronavirus, TGEV and mouse hepatitis virus.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.