Technical Data
S0098-24A
SARS, Spike Protein (Severe Acute Respiratory Syndrome, SARS-CoV, SARS Associated Coronavirus)
Description:
It has recently been shown that SARS (severe acute respiratory syndrome) is caused by a human coronavirus. Human coronaviruses are the major cause of upper respiratory tract illness, such as the common cold, in humans. Coronaviruses are positive-stranded RNA viruses, featuring the largest viral RNA genomes known toDte (27-31kb). The first step in coronavirus infection is binding of the viral spike protein, a 139kD protein, to certain receptors on host cells. The spike protein is the main surface antigen of the coronavirus. The glycosilated spike protein (as well as the nucleocapsid protein) can be detected in infected cell culture supernatants with antisera from SARS patients.

Applications:
Suitable for use in Western Blot. Other applications not tested.

Recommended Dilution:
Western Blot: 0.5-2ug/ml
Optimal dilutions to be determined by the researcher.

Positive Control:
Transfected mouse melanoma cell lysate

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgG3,k11E344Purified
SizeStorageShippingSourceHost
100ug4C Do Not FreezeBlue IceMouse
Concentration:
~0.5mg/ml
Immunogen:
Synthetic peptide corresponding to aa1124-1140 of SARS-Spike protein.
Purity:
Purified
Form
Supplied as a liquid in PBS, 0.2% gelatin, 0.05% sodium azide.
Specificity:
Recognizes SARS spike protein.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Krokhin O, Li Y, Andonov A, Feldmann H, Flick R, Jones S, Stroeher U. Mol Cell Proteomics. May;2(5):346-356 (2003). 2. Snijder,E.J., Bredenbeek,P.J., Dobbe,J.C., Thiel,V., Ziebuhr,J., et al. J. Mol. Biol. 331 (5), 991-1004 (2003). 3. Marra MA, Jones SJ, Astell CR, Holt RA, Brooks-Wilson A, Butterfield YS, Khattra J, Asano JK, Barber SA, Chan SY, et al. Science. 300(5624):1399-1404 (2003). 4. Rota PA, Oberste MS, Monroe SS, Nix WA, Campagnoli R, et al. Science. 300(5624):1394-1399 (2003).