Technical Data
S0098-37
SARS, Spike Protein (Severe Acute Respiratory Syndrome, SARS-CoV, SARS Associated Coronavirus)
Description:
A novel coronavirus has recently been identified as the causative agent of SARS (Severe Acute Respiratory Syndrome) (1-2). Coronaviruses are a major cause of upper respiratory diseases in humans (3). The genomes of these viruses are positive-stranded RNA approximately 27-31kb in length. SARS infection can be mediated by the binding of the viral spike protein, a glycosylated 139kD protein and the major surface antigen of the virus, to the angiotensin-converting enzyme 2 (ACE2) on target cells. This binding can be blocked by a soluble form of ACE2 (4).

Applications:
Suitable for use in ELISA. Other applications not tested.

Recommended Dilution:
ELISA: Detects 10ng of free peptide at 1ug/ml.
Optimal dilution determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
100ug4°C (-20°C Glycerol)Blue IceRabbit
Concentration:
~1mg/ml
Immunogen:
Synthetic peptide corresponding to amino acids 426-441 of the SARS Spike glycoprotein (Genbank accession no. P59594)
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide.
Specificity:
Recognizes SARS Spike protein (IN1).
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Marra MA, Jones SJ, Astell CR, et al. The Genome sequence of the SARS-associated corona virus. Science 2003;300:1399-404. 2. Rota PA, Oberste MS, Monroe SS, et al. Characterization of a novel coronavirus associated with severe acute respiratory syndrome. Science 2003;300:1394-9. 3. Navas-Nartin SR and Weiss S. Coronavirus replication and pathogenesis: Implications for the recent outbreak of severe acute respiratory syndrome (SARS), and the challenge for vaccine development. J Neurovirol. 2004;10:75-85. 4. Li W, Moore MJ, Vasileva N, et al. Angiotensin-converting enzyme 2 is a functional receptor for the SARS coronavirus. Nature 2003;426:450-4.