Technical Data
S0099-01D
Scavenger Receptor BI (Scavenger Receptor Class B Member 1, Scavenger Receptor Class B Type 1, Scavenging Receptor SR-BI, SCARB1, SRB1, SRBI, SR-BI, CD36 and LIMPII Analogous 1, CD36 Antigen-like 1, CD36L1, CLA1, CLA-1, Collagen Type I Receptor, MGC138242
Description:
High density lipoproteins (HDLs) play a critical role in cholesterol metabolism and their plasma concentrations are inversely correlated with risk for atherosclerosis. The SR-BI binds HDLs and mediates selective uptake of HDL cholesteryl ester. SR-BI binds HDL with high affinity, is expressed primarily in liver and nonplacental steroidgenic tissues, and mediates selective cholesterol uptake by a distinct mechanism.
In mice, it seems that SR-BI plays a key role in determining the levels of plasma lipoprotein cholesterol and the accumulation of cholesterol stores in the adrenal gland.

Applications:
Suitable for use in Immunofluorescence and Western Blot. Other applications not tested.

Recommended Dilution:
Immunofluorescence (IC): 1:50
Western Blot: 1:500 detects a band at ~82kD
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C. For long-term storage, aliquot and store at 4C. Do not freeze. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
100ul4C Do Not FreezeBlue IceMouseGoat
Concentration:
~3mg/ml
Immunogen:
Synthetic peptide corresponding to aa 400-509 from the C-terminal region of mouse Scavenger Receptor BI. Cellular Localization: Cell Membrane; multi-pass membrane protein.
Purity:
Purified by Immunoaffinity chromatography.
Form
Supplied as a liquid in Tris-citrate/phosphate, pH 7.5, 0.1% sodium azide.
Specificity:
Recognizes mouse Scavenger Receptor BI. Species Crossreactivity: human, mustelid, rat, mink, squirrel and hamster.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Bocharov, A.V., et al. Targeting of scavenger receptor class B type I sunthetic amphipathic a-helical containing peptides clocks LPS uptake and LPS-induced proinflammatory cytokine responses in THP-1 monocyte cells. J. Biol. Chem. 279(34):36072-36082, 2004. (Human)2 . Akpovi, C.D., et al. Predominance of one of the SR-BI isoforms is associated with increased esterified cholesterol levels not apoptosis in mink testis. J. Lipid Res. Vol. 47, 2006, pp. 2233-2247. 3. Akpovi, Casimir D.., et al. Research Articles: The predominance of one of the SR-BI isoforms is associated with increased esterified cholesterol levels not apoptosis in mink testis. 47: 2233-2247, 2006.